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目的:建立测定不同产地野菊花中微量元素含量的方法。方法:采用石墨炉原子吸收分光光度法(GFAAS)测定药材中铅(Pb)、镉(Cd)、砷(As)的含量;采用原子冷吸收分光光度法(HGAAS)测定药材中汞(Hg)的含量;采用火焰原子吸收分光光度法(FAAS)测定药材中铜(Cu)、铁(Fe)、锰(Mn)、钙(Ca)、锰(Mg)、锌(Zn)的含量。结果:Pb、Cd、As、Hg、Cu、Fe、Mn、Ca、Mg、Zn检测质量浓度线性范围分别为0~50(r=0.999 9)、0~10(r=0.999 2)、0~50(r=0.999 0)、0~20(r=0.999 5)、0~5(r=0.999 3)、0~15(r=0.999 8)、0~2(r=0.999 9)、0~50(r=1.000 0)、0~20(r=0.999 9)、0~2 μg/L(r=0.999 8);精密度、稳定性、重复性试验的RSD<3%;加样回收率分别为94.25%~97.43%(RSD=1.07%)、94.97%~99.46%(RSD=1.68%)、96.25%~99.46%(RSD=1.09%)、96.61%~99.91%(RSD=1.26%)、94.11%~98.41%(RSD=1.68%)、93.11%~99.59%(RSD=2.73%)、93.11%~99.48%(RSD=2.63%)、93.01%~99.85%(RSD=2.49%)、95.13%~99.75%(RSD=1.58%)、94.08%~97.37%(RSD=1.18%),n均为6。结论:该方法操作简便、稳定、重复性好,可用于不同产地野菊花中微量元素含量的测定。
OBJECTIVE:To establish a method for the contents determination of microelements in Chrysanthemum indicum from different production fields. METHODS:Graphite furnace atomic absorption (GFAAS) was used to determine the contents of Pb, Cd and As; HGAAS was used to determination the content of Hg; and FAAS was used to determine the contents of Cu, Fe, Mn, Ca, Mg and Zn. RESULTS: The linear range was 0-50 μg/L for Pb(r=0.999 9), 0-10 μg/L for Cd(r=0.999 2),0-50 μg/L for As (r=0.999 0),0-20 μg/L for Hg(r=0.999 5),0-5 μg/L for Cu(r=0.999 3), 0-15 μg/L for Fe(r=0.999 8),0-2 μg/L for Mn(r=0.999 9),0-50 μg/L for Ca(r=1.000 0),0-20 μg/L for Mg(r=0.999 9) and 0-2 μg/L for Zn(r=0.999 8); RSDs of precision, stability and reroducibility tests were lower than 3%; recoveries were 94.25%~97.43%(RSD=1.07%)、94.97%~99.46%(RSD=1.68%)、96.25%~99.46%(RSD=1.09%,n=6)、96.61%~99.91%(RSD=1.26%,n=6)、94.11%~98.41%(RSD=1.68%,n=6)、93.11%~99.59%(RSD=2.73%,n=6)、93.11%~99.48%(RSD=2.63%,n=6)、93.01%~99.85%(RSD=2.49%,n=6)、95.13%~99.75%(RSD=1.58%,n=6)、94.08%~97.37%(RSD=1.18%,n=6), respectively. CONCLUSIONS:The method is simple, stable and reproducible, and can be used for the contents determination of microelements in C. indicum from different production fields.
野菊花微量元素原子吸收分光光度法石墨炉原子吸收分光光度法原子冷吸收分光光度法火焰原子吸收分光光度法
Chrysanthemum indicumMicroelementAtomic absorption spectrometryGraphite furnace atomic absorption spectrometryAtomic cooling absorption spectrometryFlame atomic absorption spectrometry
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