OBJECTIVE：To establish the quality standard for Chaige ganmao mixture. METHODS：TLC was conducted to identify the Scutellaria baicalensis and Paeonia lactiflora；HPLC was conducted to determine the content of puerarin. The column was Zorbax Eclipse XDB-C18 with mobile phase of methanol-water（25 ∶ 75，V/V）at flow rate of 1.0 ml/min，detection wavelength was 250 nm，and column temperature was 25 ℃，and the injection volume was 5 μl. RESULTS：TLC showed clear spots and good separation. The linear range of puerarin was 18-198 μg/ml（r＝0.999 9）；RSDs of precision，stability and reproducibility tests were lower than 2％，recovery was 99.04％-99.75％（RSD＝0.28％，n＝6）. CONCLUSIONS：The standard can be used for the quality control of Chaige ganmao mixture.