OBJECTIVE：To establish a method for the simultaneous determination of chlorogenic acid， paeoniflorin， ferulic acid， berberine hydrochloride and baicalin in Lianqiao baidu pill. METHODS：HPLC was performed on the column of Agilent Eclipse XDB-C18 with mobile phase of 0.5％ phosphoric acid （triethylamine adjusted to pH 3.0）-methanol（gradient elution） at a flow rate of 1.0 ml/min， the column temperature was 30 ℃， the detection wavelength was 230 nm（for paeoniflorin and berberine hydrochloride）， 324 nm （for chlorogenic acid and ferulic acid） and 278 nm （for baicalin）， and the injection volume was 5 μl. RESULTS：The linear range was 1.884-30.144 μg/ml for chlorogenic acid（r＝0.999 9），0.392-6.272 μg/ml for paeoniflorin（r＝0.999 7）， 0.102-1.632 μg/ml for ferulic acid（r＝0.999 7），1.326-21.216 μg/ml for berberine hydrochloride（r＝0.999 8） and 1.95-31.2 μg/ml for baicalin（r＝0.999 9）， respectively； RSDs of precision， stability and reproducibility tests were lower than 2.0％； recoveries were 99.58％-102.47％（RSD＝0.93％，n＝9）， 99.21％-102.18％（RSD＝0.90％，n＝9），98.28％-101.23％（RSD＝0.86％，n＝9）， 99.66％-101.84％ （RSD＝0.82％，n＝9） and 99.18％-101.05％（RSD＝0.62％，n＝9）. CONCLUSIONS：The method is accurate， reliable， simple and fast， and can be used for the simultaneous determination of chlorogenic acid， paeoniflorin， ferulic acid， berberine hydrochloride and baicalin Lianqiao baidu pill.