OBJECTIVE：To establish a method for the identification of Chongqing local medicinal material Heracleum hemsleyanum. METHODS：The original plant morphology，morphological characteristics，microscopic characteristics （transverse section and powder），were identified. RESULTS：H. hemsleyanum had cone shape root，brown，many-branched with special fragrance；the microscopic characteristics of taproot a transverse showed no differences with lateral root transverse section，primary xylem was diarch， secondary phloem was more developed than secondary xylem，secondary phloem was rich in the oil chamber. Vascular tissues contained a large number of parenchyma cells. In TLC chromatrogram，the test sample showed the same color fluorescent spots withthe substance reference of osthole and columbianadin in the corresponding locations. CONCLUSIONS：The established method is simple with high accuracy，and can be used as the basis for identification of H. hemsleyanum.