OBJECTIVE： To provide reference for the rational use of antibiotics in outpatients who had genital tract Mycoplasma infections in Yulin area. METHODS： Using the integration culture plate，19 836 outpatient’s specimens with suspected Mycoplasma infection from Yulin Maternal and Child Health Care Hospital during 2012 to 2014 were tested， and then the results of drug susceptibility test were retrospectively analyzed. RESULTS： In the total of 19 836 inspected specimens， 5 640 cases were positive with the rate of 28.4％. The positive rate of Ureaplasma urealyticum（Uu）， Mycoplasma hominis（Mh）， mixed（Uu+ Mh） infection were 88.0％， 3.8％ and 8.2％ respectively. The positive rate of male patient with Mycoplasma infection was lower than female patient（P＜0.05）. Male and female patients with positive maximum age respectively at the age of 25 to 40 （81.3％） and 20 to 35 years old （78.6％）. From 2012 to 2014， Mycoplasma showed different degree of drug resistance to 9 kinds of antibiotics. In general， the resistance rate of Uu to lincocin was close to 100％， Mh to erythromycin， roxithromycin and azithromycin were higher than 70％， and those of Uu+Mh were lower than 10％ only to josamcine， minocycline and doxycycline. CONCLUSIONS： During 2012 to 2014， the rate of genital tract Mycoplasma infection in male and female outpatients of Yulin region increased year by year， and the infection mainly caused by Uu with a serious drug resistance. We should also pay attentions to the increase of Mh infection and mixed infection. Josamycin， minocycline and doxcycline can be used as a drug choice for empiric preferred treatment， and other antibiotics should be used based on antibiotics susceptibility test results.