OBJECTIVE：To establish the quality standard of Liyan mixture. METHODS：TLC was conducted to identify the Scrophularia ningpoensis，Terminalia chebula and Glycyrrhiza uralensis；HPLC was conducted to determine the content of chlorogenic acid. The column was Agilent Eclipse Plus C18 with mobile phase of methanol-0.2％ phosphoric acid（18 ∶ 82，V/V）at flow rate of 1.0 ml/min，the detection wavelength was 327 nm，column temperature was 30 ℃ and the injection volume was 20 μl. RESULTS： TLC showed S. ningpoensis，T. chebula and G. uralensis had clear spots and good separation. There was no interference for negative control. The linear range of chlorogenic acid was 0.16-1.6 μg（r＝0.999 9）；RSDs of precision，stability and reproducibility tests were lower than 1.0％，recovery was 96.9％-101.4％（RSD＝1.17％，n＝9）. CONCLUSIONS：The method is simple and reproducible，and can be used for the quality control of Liyan mixture.