OBJECTIVE：To optimize the extraction technology of Paeonia lactiflora. METHODS：The surface figure made by mixed uniform design combined with the quadratic polynomial stepwise regression equation is adopted to optimize the extraction technology of P. lactiflora by using the content of paeoniflorin as index，with extraction solvent，the amount of extraction solvent， extraction time，extraction times as factors. RESULTS：The optimal extraction technology of P. lactiflora was as follows as 90％ ethanol，12-folds extraction solvent，extracting for 150 min，extracting for 2 times. The measured value of 3 validation tests were 2.848 4％，2.795 7％，2.841 9％（RSD＝0.82％，n＝3），which was close to the predicted value 2.848 4％. CONCLUSIONS：The method is convenient and accurate，and can be used for the extraction of P. lactiflora.