OBJECTIVE：To establish a method for simultaneous determination of puerarin，berberine hydrochloride and baicalin in Erxieting syrup. METHODS：HPLC was performed on the column of Kromasil-C18 with mobile phase of methanol-0.2％ Phosphoric acid solution（gradient elution）at flow rate of 1.0 ml/min，column temperature was 30 ℃，detection wavelength was 275 nm，and the volume injection was 10 μl. RESULTS：The linear was 10.43-83.44 μg/ml for puerarin，10.71-85.68 μg/ml for berberine hydrochloride and 12.58-100.64 μg/ml for baicalin（r≥0.999 3）；RSDs of precision，stability and reproduciblity tests were lower than 2％；recoveries were 98.25％-100.87％（RSD＝0.92％，n＝6），99.41％-101.40％（RSD＝0.68％，n＝6）and 98.13％-99.76％ （RSD＝0.57％，n＝6），respectively. CONCLUSIONS：The method is simple，reliable，can be used for the quality control of Erxieting syrup.