OBJECTIVE：To establish a method for content determination of astragaloside in Danggui buxue formula granule， and investigate the influence of different conditions of dissolution on the content. METHODS：HPLC was performed on the column of Aglient C18 with mobile phase of acetonitrile-water（28 ∶72，V/V）at flow rate of 1.0 ml/min，column temperature was 25 ℃，and the injection volume was 10 μl. RESUITS：The linear range of astragaloside was 2.91-17.46 μg；RSDs of precision，stability were lower than 2％ ，and reproducibility tests was 3.63％ ，recovery was 95.76％ -98.20％（RSD＝1.03％ ，n＝6）. The optimized use method was mixing the two granules with 80 ℃ hot water and keeping heat preservation for 5 min successively before taken. CONCLUSIONS： The method is simple and reproducible，and can be used for the content determination of astragaloside in Danggui buxue formula granule.