OBJECTIVE: To study the germination and seedling enzymatic activity of Uygur medicine Capparis spirrosa seeds, and to provide reference for improving the quality of seedlings. METHODS: The germination test was carried out under different soaking conditions, i.e. 10% potassium permanganate for 30 min, concentrated sulfuric acid for 30 min, 100 ℃ hot water for 30 min, 75% alcohol for 30 min. The germination percentage, germination potential, germination index, activity index and length of radicel in seeds, the activity of SOD and POD in fresh leaves, were all determined, as well as the weight of 10 plants after dried. Those indicators were compared with those of control group (no soaking treatment). RESULTS: Compared with control group, germination percentage, germination potential, germination index, activity index and length of radicel of seeds soaked in concentrated sulfuric acid for 30 min were the highest (P<0.05); the activity of SOD and POD in fresh leaves of seedlings and the weight of dried substance were also in high level. CONCLUSIONS: It can promote the best soaking condition of C. spirrosa seeds is to soak seed in concentrated sulfuric acid for 30 min. The trial result provides reference for the C. spirrosa artificial cultivation.