OBJECTIVE：To establish a method for the simultaneous determination of chlorogenic acid and vitexin in Lophatherum gracile. METHODS： HPLC was performed on the column was Waters Atlantis C18 with mobile phases of acetonitrile - water （gradient elution） at a flow rate of 1.0 ml/min， detection wavelength was 280 nm， column temperature was 35 ℃， and the injection volume was 10 μl. RESULTS：The linear range was 0.041 0-1.228 8 μg for chlorogenic acid （r＝0.999 8） and 0.264 0-7.920 0 μg for vitexin（r＝0.999 9）； RSDs of precision， stability and reproducibility tests were lower than 2％；recoveries were 97.6％-102.3％ （RSD＝1.85％， n＝9） and 97.1％-101.3％（RSD＝1.19％，n＝9）， respectively. CONCLUSIONS： The method is simple， stable and reproducible， and can be used for the simultaneous determination of chlorogenic acid and vitexin in L. gracile.