OBJECTIVE： To study the correlation between antibacterials amount and drug resistance of Echerichia coli， and to provide reference for clinical use of antibacterials. METHODS： Retrospective review was used to calculate DDDs of antibacterials and resistance rate of Escherichia coli to 11 kinds of antibacterials each quarter. The correlation analysis was carried out using the SPSS 13.0 statistical software. RESULTS： The resistance rates of E. coli to piperacillin/tazobactam， cefoperazone/sulbactam and levofloxacin were with upward trends， and the others showed downward trends. The resistance rates of E. coli to meropenem and imipenem/cilastatin appeared in 2014， increasing from 0 to 8.8％ and 9.4％， respectively. DDDs of them were significantly correlated to drug resistance of E. coli， showing positive correlation （r＝0.915， 0.793， P＜0.01）. DDDs of piperacillin/tazobactam was significantly correlated to resistance rate of E. coli（r＝0.807，P＜0.01），while that of ceftazidime was negatively correlated to resistance rate of E. coli （r＝－0.672，P＜0.05）. There was no statistical significance in resistance rate of E. coli to other 7 kinds of antibacterials. CONCLUSIONS： There are some correlations between the DDDs and resistance rates. We should strengthen the monitoring of bacterial resistance and the management of  rational application of antibacterials.