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硫化氢对心肌成纤维细胞增殖的抑制作用及机制研究
Study on inhibitory effect and mechanism of hydrogen sulfide on the proliferation of cardiac fibroblasts
- 中国药房 2023年34卷第4期 页码：438-443
- 作者机构：
1.南通大学附属妇幼保健院/南通市儿童医院药学部，江苏 南通 226007
2.南通大学药学院，江苏 南通 226001
3.南通大学附属妇幼保健院/南通市儿童医院心电图室，江苏 南通 226007
- 作者简介：
主管药师，硕士。研究方向：临床药学、药理学。E-mail：guiqulaixi214@163.com
主治医师。研究方向：心血管疾病的临床治疗。E-mail：lulu920317@163.com
- 基金信息：江苏省333高层次人才培养工程[No.（2022）3-16-670号];南通市科技计划（指导性）项目(JCZ20167);南通市卫生健康委员会科研课题(QA2020034)
DOI：10.6039/j.issn.1001-0408.2023.04.11
中图分类号： R965纸质出版日期：2023-02-28，
收稿日期：2022-09-07，
修回日期：2022-12-19，
扫 描 看 全 文
刘露露,秦燕,孟国梁等.硫化氢对心肌成纤维细胞增殖的抑制作用及机制研究 Δ[J].中国药房,2023,34(04):438-443.
LIU Lulu,QIN Yan,MENG Guoliang,et al.Study on inhibitory effect and mechanism of hydrogen sulfide on the proliferation of cardiac fibroblasts[J].ZHONGGUO YAOFANG,2023,34(04):438-443.
刘露露,秦燕,孟国梁等.硫化氢对心肌成纤维细胞增殖的抑制作用及机制研究 Δ[J].中国药房,2023,34(04):438-443. DOI： 10.6039/j.issn.1001-0408.2023.04.11.
LIU Lulu,QIN Yan,MENG Guoliang,et al.Study on inhibitory effect and mechanism of hydrogen sulfide on the proliferation of cardiac fibroblasts[J].ZHONGGUO YAOFANG,2023,34(04):438-443. DOI： 10.6039/j.issn.1001-0408.2023.04.11.
摘要
目的
2
研究硫化氢（H
2
S）对心肌成纤维细胞增殖的抑制作用及机制。
方法
2
取新生SD雄性大鼠心脏，采用差速离心法分离心肌成纤维细胞。以硫氢化钠作为H
2
S的供体，检测H
2
S对血管紧张素Ⅱ（Ang Ⅱ）诱导心肌成纤维细胞增殖、羟脯氨酸含量以及去乙酰化酶3（SIRT3）蛋白表达的影响。用干扰RNA技术下调SIRT3表达后，观察H
2
S对Ang Ⅱ诱导的心肌成纤维细胞增殖、羟脯氨酸的含量以及Ⅰ型胶原（Col Ⅰ）、Ⅲ型胶原（Col Ⅲ）和视神经萎缩蛋白1（OPA1）表达的影响。
结果
2
H
2
S可抑制Ang Ⅱ诱导的心肌成纤维细胞增殖，降低羟脯氨酸含量，增强SIRT3蛋白表达（
P
＜0.05）。用干扰RNA技术下调SIRT3表达后，H
2
S对Ang Ⅱ诱导的心肌成纤维细胞增殖抑制作用、羟脯氨酸含量降低作用均被抑制，且H
2
S降低Col Ⅰ、Col Ⅲ表达的作用和增强OPA1表达的作用也明显减弱。
结论
2
H
2
S依赖增加SIRT3表达来抑制Ang Ⅱ诱导的心肌成纤维细胞增殖。
Abstract
OBJECTIVE
2
To investigate the inhibitory effect and the possible mechanism of hydrogen sulfide （H
2
S） on the proliferation of cardiac fibroblasts.
METHODS
2
The heart of neonatal SD rats was collected， and cardiac fibroblasts were separated with differential centrifugation. Using sodium hydrosulfide as the donor of H
2
S， the effects of H
2
S on the proliferation of cardiac fibroblasts induced by angiotensin Ⅱ （Ang Ⅱ）， hydroxyproline content and the expression of sirtuin 3 （SIRT3） protein were detected. After SIRT3 knockdown with siRNA technology， the effects of H
2
S on the proliferation of cardiac fibroblasts induced by Ang Ⅱ， hydroxyproline content， the expressions of collagen Ⅰ （Col Ⅰ）， collagen Ⅲ （Col Ⅲ ） and optic atrophy protein 1 （OPA1） were detected.
RESULTS
2
H
2
S could inhibit the proliferation of Ang Ⅱ-induced cardiac fibroblasts， reduce the content of hydroxyproline and increase the expression of SIRT3 （
P
＜0.05）. After down-regulating the expression of SIRT3 with siRNA technology， the inhibition of H
2
S on the proliferation of Ang Ⅱ-induced cardiac fibroblasts and the reduction of hydroxyproline content were both inhibited， and the effect of H
2
S on reducing the expression of Col Ⅰ and Col Ⅲ and enhancing the expression of OPA1 was also significantly weakened.
CONCLUSIONS
2
H
2
S inhibits the proliferation of Ang Ⅱ-induced cardiac fibroblasts through increasing the expression of SIRT3.
关键词
硫化氢心肌成纤维细胞血管紧张素Ⅱ去乙酰化酶3
Keywords
cardiac fibroblastsangiotensin Ⅱsirtuin 3
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