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1.河北中医学院药学院,石家庄 050200
2.河北中医学院河北省中药炮制技术创新中心,石家庄 050091
3.河北省中药资源利用与质量评价国际联合研究中心,石家庄 050091
4.河北中医学院实验中心,石家庄 050200
5.河北化工医药职业技术学院,石家庄 050026
硕士研究生。研究方向:中药炮制与资源。E-mail:yangzhenmin89@126.com
教授,博士生导师,博士。研究方向:中药资源。 E-mail:zwhjzs@126.com
纸质出版日期:2023-06-15,
收稿日期:2023-01-12,
修回日期:2023-04-02,
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杨震旻,李冰,毛福英等.北沙参茎叶和根中挥发油的提取与抗真菌活性研究 Δ[J].中国药房,2023,34(11):1358-1362.
YANG Zhenmin,LI Bing,MAO Fuying,et al.Study on the extraction and antifungal activity of volatile oil from stems, leaves and roots of Glehnia littoralis[J].ZHONGGUO YAOFANG,2023,34(11):1358-1362.
杨震旻,李冰,毛福英等.北沙参茎叶和根中挥发油的提取与抗真菌活性研究 Δ[J].中国药房,2023,34(11):1358-1362. DOI: 10.6039/j.issn.1001-0408.2023.11.14.
YANG Zhenmin,LI Bing,MAO Fuying,et al.Study on the extraction and antifungal activity of volatile oil from stems, leaves and roots of Glehnia littoralis[J].ZHONGGUO YAOFANG,2023,34(11):1358-1362. DOI: 10.6039/j.issn.1001-0408.2023.11.14.
目的
2
探究北沙参茎叶和根中挥发油的提取工艺,解析北沙参茎叶和根中挥发油所含化学成分,并初步评价其体外抗真菌活性。
方法
2
以水蒸气蒸馏法为基础,通过单因素与正交实验优化北沙参茎叶和根中挥发油提取方法,利用气相色谱-质谱联用(GC-MS)技术鉴定北沙参茎叶和根中挥发油所含化学成分并计算其相对百分含量。采用纸片扩散法测定北沙参茎叶和根中挥发油对茄病镰刀菌、半裸镰刀菌、尖孢镰刀菌、寄生曲霉和黄曲霉的抗真菌活性。
结果
2
北沙参最佳提取工艺为料液比1∶15,蒸馏时间5 h,KCl浓度15%。从北沙参茎叶挥发油中鉴定出11种成分,根挥发油中共鉴定出8种成分。人参炔醇为北沙参茎叶挥发油和根挥发油的共有成分,其在茎叶中的含量为38.21%,根中的含量为74.02%。北沙参茎叶挥发油和根挥发油对茄病镰刀菌、半裸镰刀菌、尖孢镰刀菌、寄生曲霉和黄曲霉均有一定的抑制作用,以茎叶挥发油较强。
结论
2
北沙参茎叶挥发油和根挥发油的化学成分差异明显,但二者均具有一定的体外抗真菌活性。
OBJECTIVE
2
To explore the extraction process of volatile oil from the stems, leaves and roots of
Glehnia littoralis
, analyze the chemical components of the volatile oil from the stems, leaves and roots of
G. littoralis
, and preliminarily evaluate its
in vitro
antifungal activity.
METHODS
2
Based on the steam distillation method, single factor test and orthogonal experiment were conducted to optimize the extraction method of volatile oil from the stems, leaves and roots of
G. littoralis
. The chemical components of the volatile oil from the stems, leaves and roots of
G. littoralis
were identified by using gas chromatography-mass spectrometry (GC-MS) technology and their relative contents were calculated. The antifungal activity of volatile oils from the stems, leaves and roots of
G. littoralis
against
Fusarium solani, Fusarium incarnatum, Fusarium oxysporum, Aspergillus parasiticus
and
Aspergillus flavus
was determined by paper diffusion method.
RESULTS
2
The optimal extraction process of
G. littoralis
was solid-liquid ratio of 1∶15, distillation time of 5 hours, and KCl concentration of 15%. Eleven components were identified from the volatile oil of the stems and leaves of
G. littoralis
, and a total of eight components were identified from the volatile oil of the roots. Ginsenethinol was a common component in the volatile oil from the stems, leaves and roots of
G. littoralis
, its contents in the stems and leaves, roots were 38.21% and 74.02%, respectively. The volatile oil from the stems, leaves and roots of
G. littoralis
had a certain inhibitory effect on
F. solani, F. incarnatum, F. oxysporum, A. parasiticus and A. flavus
, especially volatile oil from the stems and leaves.
CONCLUSIONS
2
There is a significant difference in chemical components of the volatile oil between the roots, stems and leaves of
G. littoralis
, both of which have certain
in vitro
antifungal activity.
北沙参茎叶根挥发油真菌抗真菌活性化学成分
stemsleavesrootsvolatile oilfungusantifungal activitychemical components
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