OBJECTIVE

2

To study the quality of

Codonopsis pilosula

with different commodity specification grades， and to provide the data support for market transactions， scientific research and clinical use.

METHODS

2

According to the classification standard of commodity specification grades of

C. pilosula

， 17 batches of

C. pilosula

from different producing areas， origins and commodity specification grades were collected. The contents of tangshenoside Ⅰ， lobetyolin and atractylenolide Ⅲ were determined by HPLC. The contents of alcohol-soluble extracts were determined by hot dipping method stated in general rule 2201 of

Chinese Pharmacopeia

（part Ⅳ）. The contents of polysaccharide were determined by phenol-sulfuric acid method （calculated by D-glucose anhydrous）.

RESULTS

2

For cultivar of

C. pilosula

， four specifications and three commodity grades of

C. pilosula

all contained tangshenoside Ⅰ and lobetyolin； Radix

C. pilosula

from Shanxi of China and

C. pilosula

from Wenxian County of China， also contained atractylenolide Ⅲ. In terms of the contents of tangshenoside Ⅰ， lobetyolin and atractylenolide Ⅲ， the content of second class was equivalent to that of first class， even better than the first class， while the content of third class was lower than that of first class and second class； the content of tangshenoside Ⅰ was the highest among the two types of wild

C. pilosula

. The contents of alcohol-soluble extracts and polysaccharides in first class cultivated

C. pilosula

were higher than those of second class， and the second class was higher than the third class； wild

C. pilosula

had low content of alcohol-soluble extracts and polysaccharides.

CONCLUSIONS

2

The internal quality of

C. pilosula

is basically consistent with the classification standard of different commodity specification grades； the content of each indicator in first-class and second-class medicinal herb is high， making them high-quality medicinal herbs.