OBJECTIVE

2

To screen the differential components of coumarins in

Angelica dahurica

from two origins （

A. dahurica

cv. ‘Hangbaizhi’；

A. dahurica

cv. ‘Qibaizhi’）.

METHODS

2

Non-targeted metabolomics technique of UPLC-Q-Exactive-MS/MS was used to analyze the coumarins in 6 batches of

A. dahurica

cv. ‘Hangbaizhi’ and 12 batches of

A. dahurica

cv. ‘Qibaizhi’. The differential components were screened by principal component analysis， partial least squares discriminant analysis and orthogonal partial least squares discriminant analysis. Cluster analysis was performed on differential components.

RESULTS

2

A total of 41 coumarins were identified in 18 batches of samples， in which 23 coumarins were differential components. Therein， 6 differential components were higher in content in

A. dahurica

cv. ‘Hangbaizhi’， while 17 differential components were higher in content in

A. dahurica

cv. ‘Qibaizhi’. The content of marmesin galactoside in

A. dahurica

cv. ‘Hangbaizhi’ was significantly higher than that in

A. dahurica

cv. ‘Qibaizhi’. Based on 23 differential components，

A. dahurica

cv. ‘Hangbaizhi’ and

A. dahurica

cv. ‘Qibaizhi’ could be grouped into one category， respectively.

CONCLUSIONS

2

The screened differential components of coumarins can be used to distinguish

A. dahurica

cv. ‘Hangbaizhi’ from

A. dahurica

cv. ‘Qibaizhi’， especially marmesin galactoside contributed the most， which can be used to identify

A. dahurica

cv. ‘Hangbaizhi’ and

A. dahurica

cv. ‘Qibaizhi’.