OBJECTIVE

2

To study the pharmacokinetics of paeoniflorin， hesperidin， naringenin， formononetin and enoxolone from Chitong xiaoyanling

granules in rats.

METHODS

2

Six male SD rats fasted but not deprived of water for 12 h were given

Chitong xiaoyanling granules （5.0 g/kg） intragastrically at one time. Blood was collected from inner canthus of rats at different time points after administration. Plasma samples were pretreated with acetonitrile precipitated protein （sulfamethoxazole as internal standard）， and the concentrations of 5 ingredients in plasma were determined by liquid chromatography-tandem mass spectrometry （LC-MS/MS）. The pharmacokinetic parameters of each ingredient were calculated using DAS 3.0 software.

RESULTS

2

After intragastric administration of Chitong xiaoyanling granules，

t

max

and

t

1/2

of formononetin were the shortest among 5 ingredients （0.25 h and 0.39 h， respectively）. For naringenin and enoxolone， the

t

max

was shorter， but

t

1/2

was longer， and there was an obvious double-peak phenomenon in the drug-time curve of naringenin. Compared with the other three components，

c

max

and AUC of paeoniflorin and hesperidin were not directly proportional to the content of

in vitro

components.

CONCLUSIONS

2

Formononetin， naringin and enoxolone in Chitong xiaoyanling granules were absorbed rapidly in rats， while paeoniflorin and hesperidin were absorbed slowly.