OBJECTIVE

2

To establish the fingerprints of

Ardisia crenata

，

Sophora tonkinensis

and their couplet medicines， and to determine the contents of five components in them.

METHODS

2

Using water as solvent， single lyophilized powder of

A. crenata

and

S. tonkinensis

and combined lyophilized powder of their couplet medicines were prepared by combining lyophilization technology. The fingerprints of three lyophilized powder samples were established by using high-performance liquid chromatography （HPLC）， and the contents of 5 kinds of components such as gallic acid were determined simultaneously.

RESULTS

2

There were 5， 10 and 14 common peaks in the fingerprints for single lyophilized powder of

A. crenata

and

S. tonkinensis

and combined lyophilized powder of their couplet medicines； the similarities of them with the control fingerprints were all greater than 0.90. For combined lyophilized powder of couplet medicines， peak 3 was identified as gallic acid， peak 4 as matrine， peak 6 as oxymatrine， peak 8 as bergenin， and peak 14 as trifolirhizin. In single lyophilized powder of

A. crenata

， the average contents of gallic acid and bergenin were 0.499 3 and 4.962 6 mg/g， respectively. In single lyophilized powder of

S. tonkinensis

， the average contents of matrine， oxymatrine and trifolirhizin were 3.046 0， 2.336 6 and 0.278 6 mg/g， respectively. In combined lyophilized powder of couplet medicines， the average contents of gallic acid， matrine， oxymatrine， bergenin and trifolirhizin were 0.560 6， 2.548 7， 1.382 2， 5.960 7 and 0.279 1 mg/g， respectively. The transfer rates were 8.87%-513.19%.

CONCLUSIONS

2

The established fingerprint and content determination methods are stable and feasible， and can be used for the quality control of

A. crenata

and

S. tonkinensis

and their couplet medicines. The average contents of matrine and oxymatrine in combined lyophilized powder of

A. crenata

-

S. tonkinensis

couplet medicines are decreased.