艾纳香中艾纳香素的提取工艺优化及抗菌消炎活性评价

齐维金; 刘瑞秀; 潘淑娟; 何贤芳; 王鸿颖; 王鲁

doi:10.6039/j.issn.1001-0408.2023.19.04

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艾纳香中艾纳香素的提取工艺优化及抗菌消炎活性评价

药学研究 | 更新时间：2023-10-09

- 艾纳香中艾纳香素的提取工艺优化及抗菌消炎活性评价
- Optimization of extraction process and evaluation of the antibacterial and anti-inflammatory activity of blumeatin from Blumea balsamifera
- 中国药房 2023年34卷第19期页码：2323-2327
- 作者机构：
  
  1.贵州大学药学院，贵阳　550025
  2.贵州大学西南特色药用生物资源开发利用教育部工程研究中心，贵阳　550025
- 作者简介：
  
  硕士。研究方向：天然药物成分及生理活性。E-mail：2038298834@qq.com
  教授，硕士生导师，博士。研究方向：药理学。E-mail：wanglu7007@163.com
- 基金信息：
  
  国家自然科学基金项目(32160849)
- DOI：10.6039/j.issn.1001-0408.2023.19.04
  中图分类号： R284.2;R965
- 纸质出版日期：2023-10-15，
  
  收稿日期：2023-03-29，
  
  修回日期：2023-09-11，
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齐维金,刘瑞秀,潘淑娟等.艾纳香中艾纳香素的提取工艺优化及抗菌消炎活性评价 ^Δ[J].中国药房,2023,34(19):2323-2327.

QI Weijin,LIU Ruixiu,PAN Shujuan,et al.Optimization of extraction process and evaluation of the antibacterial and anti-inflammatory activity of blumeatin from Blumea balsamifera[J].ZHONGGUO YAOFANG,2023,34(19):2323-2327.
齐维金,刘瑞秀,潘淑娟等.艾纳香中艾纳香素的提取工艺优化及抗菌消炎活性评价 ^Δ[J].中国药房,2023,34(19):2323-2327. DOI： 10.6039/j.issn.1001-0408.2023.19.04.

QI Weijin,LIU Ruixiu,PAN Shujuan,et al.Optimization of extraction process and evaluation of the antibacterial and anti-inflammatory activity of blumeatin from Blumea balsamifera[J].ZHONGGUO YAOFANG,2023,34(19):2323-2327. DOI： 10.6039/j.issn.1001-0408.2023.19.04.

摘要

目的

优化艾纳香中艾纳香素的提取工艺，并评价其抗菌消炎活性。

方法

利用高效液相色谱法测定艾纳香提取液中艾纳香素的含量。在单因素实验的基础上，以艾纳香素得率为评价指标，以乙醇体积分数、液料比、提取时间为考察因素，采用Box-Behnken响应面法优化艾纳香素的提取工艺并进行验证。使用微量稀释法测定艾纳香素对化脓链球菌、金黄色葡萄球菌、无乳链球菌、变异链球菌、枯草芽孢杆菌和藤黄微球菌的抗菌活性。通过小鼠耳廓肿胀实验和毛细血管通透性实验考察艾纳香素的消炎活性。

结果

最优提取工艺为：乙醇体积分数90%，液料比15∶1，以80 ℃回流提取2 h；采用该提取工艺的艾纳香素得率为1.97 mg/g。艾纳香素对化脓链球菌、金黄色葡萄球菌、无乳链球菌、变异链球菌、枯草芽孢杆菌、藤黄微球菌的最低抑菌浓度分别为50.00、200.00、400.00、400.00、800.00、1 600.00 μg/mL，对化脓链球菌、金黄色葡萄球菌的最低杀菌浓度分别为400.00、1 600.00 μg/mL；并能显著抑制二甲苯所致小鼠耳廓肿胀及醋酸所致小鼠毛细血管的通透性（

＜0.05或

＜0.01）。

结论

优化所得的艾纳香素提取工艺稳定可行；提取的艾纳香素对化脓链球菌具有一定的抗菌活性，且具有良好的消炎活性。

Abstract

OBJECTIVE

To optimize the extraction process of blumeatin from

Blumea balsamifera

and to evaluate its antibacterial and anti-inflammatory activity.

METHODS

The content of blumeatin in the extract of

B. balsamifera

was determined by HPLC. On the basis of the single factor experiment， the extraction technology of blumeatin was optimized by the Box-Behnken response surface method with the volume fraction of ethanol， liquid-solid ratio and extraction time as the factors， using the yield of blumeatin as index. Microdilution method was used to determine the antibacterial activity of blumeatin against

Streptococcus pyogenes

，

Staphylococcus aureus

，

Streptococcus agalactiae

，

Streptococcus mutans

，

Bacillus subtilis

and

Micrococcus luteus

. The anti-inflammatory activity of blumeatin was evaluated by ear swelling test and capillary permeability test in mice.

RESULTS

The optimal extraction technology was as follows： ethanol concentration of 90%， liquid-material ratio of 15∶1， extraction time of 2 h at 80 ℃； the yield of blumeatin using this extraction process was 1.97 mg/g. The minimum inhibitory concentrations of blumeatin for

S. pyogenes

，

S. aureus

，

S. agalactiae

，

S. mutans

，

B. subtilis

and

M. luteus

were 50.00， 200.00， 400.00， 400.00， 800.00 and 1 600.00 μg/mL， respectively； the minimum bactericidal concentrations of blumeatin for

S. pyogenes

and

S. aureus

were 400.00 and 1 600.00 μg/mL， respectively. Blumeatin could significantly inhibit the ear swelling induced by xylene and capillary permeability induced by acetic acid in mice（

＜0.05 or

＜0.01）.

CONCLUSIONS

The optimized extraction technology of blumeatin is stable and feasible. The extracted blumeatin has a certain antibacterial effect against

S. pyogenes

and a good anti-inflammatory activity.

关键词

艾纳香艾纳香素提取工艺响应面法消炎抗菌

Keywords

blumeatinextraction processresponse surface methodanti-inflammatoryantibacterial

references

MA J，REN Q H，DONG B J，et al. NO inhibitory consti- tuents as potential anti-neuroinflammatory agents for AD from Blumea balsamifera[J]. Bioorg Chem，2018，76：449-457.

HASEGAWA H，YAMADA Y，KOMIYAMA K，et al. Dihydroflavonol BB-1，an extract of natural plant Blumea balsamifera，abrogates TRAIL resistance in leukemia cells[J]. Blood，2006，107（2）：679-688.

OSAKI N，KOYANO T，KOWITHAYAKORN T，et al. Sesquiterpenoids and plasmin-inhibitory flavonoids from Blumea balsamifera[J]. J Nat Prod，2005，68（3）：447-449.

CHEN X，SU H Z Z. Detailed studies on the anticancer action of rosmarinic acid in human Hep-G2 liver carcinoma cells：evaluating its effects on cellular apoptosis，caspase activation and suppression of cell migration and invasion[J]. J BUON，2020，25（4）：2011-2016.

XU S B，CHEN W F，LIANG H Q，et al. Protective action of blumeatin against experimental liver injuries[J]. Acta Pharmacol Sin，1993，14（4）：376-378.

NESSA F，ISMAIL Z，KARUPIAH S，et al. RP-HPLC method for the quantitative analysis of naturally occurring flavonoids in leaves of Blumea balsamifera DC[J]. J Chromatogr Sci，2005，43（8）：416-420.

高月，王万林，谢雪艳，等. 结合体外抗菌抗炎活性评价筛选艾纳香油的提取工艺[J]. 中药材，2020，43（5）：1196-1199.

GAO Y， WANG W L， XIE X Y， et al. Screening the extraction technology of Blumea balsamifera oil based on in vitro antibacterial and anti-inflammatory activity evaluation[J]. J Chin Med Mater，2020，43（5）：1196-1199.

魏玉好，冯将，王玉坤，等. 金英黄归汤抗炎活性的评价[J]. 畜牧与兽医，2018，50（4）：138-143.

WEI Y H，FENG J，WANG Y K，et al. Assay on the anti-inflammatory activities of Jinying huanggui decoction[J]. Anim Husb Vet Med，2018，50（4）：138-143.

范东生，李玲，王可可，等. 艾纳香化学成分及药理活性的研究进展[J]. 中国药房，2022，33（10）：1274-1280.

FAN D S，LI L，WANG K K，et al. Research progress on chemical constituents and pharmacological activities of Blumea balsamifera[J]. China Pharm，2022，33（10）：1274-1280.

ZHANG Y，WU Y T，ZHENG W，et al. The antibacterial activity and antibacterial mechanism of a polysaccharide from Cordyceps cicadae[J]. J Funct Foods，2017，38：273-279.

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