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1.湖北省中医院/湖北中医药大学附属医院/湖北省中医药研究院药事部,武汉 430061
2.湖北中医药大学药学院,武汉 430065
副主任药师,硕士。研究方向:中药的品种与质量分析。电话:027-88928141。E-mail:sweetness2001@126.com
主任药师。研究方向:医院中药制剂研发、中药饮片质量控制。电话:027-88928141。E-mail:chenshuhe606@163.com
纸质出版日期:2024-01-30,
收稿日期:2023-06-06,
修回日期:2023-12-26,
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朱田密,陈树和,严劲松等.光皮木瓜特征图谱及3种黄酮类成分含量测定研究 Δ[J].中国药房,2024,35(02):150-154.
ZHU Tianmi,CHEN Shuhe,YAN Jingsong,et al.Study on characteristic chromatogram of Chaenomeles sinensis and content determination of 3 flavones[J].ZHONGGUO YAOFANG,2024,35(02):150-154.
朱田密,陈树和,严劲松等.光皮木瓜特征图谱及3种黄酮类成分含量测定研究 Δ[J].中国药房,2024,35(02):150-154. DOI: 10.6039/j.issn.1001-0408.2024.02.05.
ZHU Tianmi,CHEN Shuhe,YAN Jingsong,et al.Study on characteristic chromatogram of Chaenomeles sinensis and content determination of 3 flavones[J].ZHONGGUO YAOFANG,2024,35(02):150-154. DOI: 10.6039/j.issn.1001-0408.2024.02.05.
目的
2
建立光皮木瓜的特征图谱,同时测定芦丁、金丝桃苷、槲皮苷3种黄酮类成分的含量,鉴别光皮木瓜和皱皮木瓜。
方法
2
采用高效液相色谱(HPLC)法。以Agilent 5 TC-C
18
为色谱柱,以乙腈-0.2%磷酸溶液为流动相进行梯度洗脱,流速为1.0 mL/min,柱温为30 ℃,特征图谱检测波长为330 nm,含量测定检测波长为350 nm。采用《中药色谱指纹图谱相似度评价系统(2012版)》建立光皮木瓜的特征图谱并评价相似度,使用SPSS 23.0软件对15批光皮木瓜(S1~S15)进行聚类分析;测定15批光皮木瓜和7批皱皮木瓜(S16~S22)中3种黄酮类成分的含量,比较二者特征图谱。
结果
2
15批光皮木瓜样品的特征图谱相似度为0.783~0.969;共确定11个特征峰;指认了4种成分,分别为绿原酸、芦丁、金丝桃苷、槲皮苷。15批光皮木瓜样品聚为2类,S5~S8为一类,其余为一类。光皮木瓜与皱皮木瓜的特征图谱有明显区别。15批光皮木瓜样品中芦丁、金丝桃苷、槲皮苷含量分别为48.99~294.45、3.49~102.55、31.98~149.49 μg/g,皱皮木瓜中芦丁含量低于光皮木瓜,且皱皮木瓜中均未检出金丝桃苷(S20样品除外)和槲皮苷。
结论
2
所建立的光皮木瓜的特征图谱和3种黄酮类成分的含量测定方法可用于其质量控制及其与皱皮木瓜的鉴别。
OBJECTIVE
2
To establish the characteristic chromatogram of
Chaenomeles sinensis
, determine the contents of rutin, hyperin and quercitrin, and to identify
C. sinensis
and
C. speciosa
.
METHODS
2
HPLC method was performed on Agilent 5 TC-C
18
column, with acetonitrile-0.2% formic acid solution as the mobile phase for gradient elution, at the flow rate of 1.0 mL/min. The column temperature was 30 ℃. The detection wavelength was 330 nm in characteristic chromatogram and 350 nm in content determination. The characteristic chromatogram of
C. sinensis
was established and similarity was evaluated by the
Similarity Evaluation System for Chromatographic Fingerprint of TCM
(2012 edition). Hierarchical cluster analysis of 15 batches of
C. sinensis
(S1-S15) was performed by using SPSS 23.0 software. The contents of 3 flavones in 15 batches of
C. sinensis
and
7 batches of
C. speciosa
(S16-S22) were determined, while their characteristic chromatograms were compared.
RESULTS
2
The similarities of the characteristic chromatogram for 15 batches of
C. sinensis
ranged from 0.783 to 0.969, and 11 characteristic peaks were confirmed. Four constituents were identified as chlorogenic acid, rutin, hyperin and quercitrin. The medicinal materials in 15 batches of
C. sinensis
could be divided into 2 categories: S5-S8 were one category, and the others belonged to one category. The characteristic chromatogram of
C. sinensis
was obviously different from
C. speciosa
. The contents of rutin, hyperin and quercitrin in 15 batches of
C. sinensis
were 48.99-294.45, 3.49-102.55, 31.98-149.49 μg/g, respectively. The content of rutin in
C. speciosa
was lower than that in
C. sinensis.
None of hyperin (except for S20) and quercitrin were detected in
C. speciosa
.
CONCLUSIONS
2
The characteristic chromatogram and the method for content determination of 3 flavones in
C. sinensis
are established successfully and can be used for the quality control of
C. sinensis
and its identification from
C. speciosa
.
光皮木瓜黄酮特征图谱高效液相色谱法
flavonescharacteristic chromatogramHPLC
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