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黄芩汤的抗须癣毛癣菌活性及作用机制研究
Study on the antifungal activity and mechanism of Huangqin decoction against
Trichophyton mentagrophytes - 中国药房 2024年35卷第3期 页码：311-315
- 作者机构：
1.江西省人民医院（南昌医学院第一附属医院）药学部，南昌;330006
2.南昌大学药学院，南昌 330006
3.江西省人民医院（南昌医学院第一附属医院）临床医学研究所，南昌 330006
4.江西中医药大学现代中药制剂教育部重点实验室，南昌 330004
- 作者简介：
主管中药师，博士。研究方向：中药新型给药系统。电话：0791-86891529。E-mail：984075899@qq.com
主任药师。研究方向：医院药学。电话：0791-86895684。E-mail：hjx0108@163.com
- 基金信息：江西省自然科学基金项目(20232BAB216124);江西省卫生健康委科技计划项目(202310011);江西省中医药中青年骨干人才（第四批）培养计划(赣中医药科教字〔2022〕7号);江西省中医药管理局中药制剂临床安全监测和转化应用重点研究室项目
DOI：10.6039/j.issn.1001-0408.2024.03.08
中图分类号： R275.9;R965纸质出版日期：2024-02-15，
收稿日期：2023-08-03，
修回日期：2023-12-22，
扫 描 看 全 文
沈成英,罗忠,章佩等.黄芩汤的抗须癣毛癣菌活性及作用机制研究 Δ[J].中国药房,2024,35(03):311-315.
SHEN Chengying,LUO Zhong,ZHANG Pei,et al.Study on the antifungal activity and mechanism of Huangqin decoction against Trichophyton mentagrophytes[J].ZHONGGUO YAOFANG,2024,35(03):311-315.
沈成英,罗忠,章佩等.黄芩汤的抗须癣毛癣菌活性及作用机制研究 Δ[J].中国药房,2024,35(03):311-315. DOI： 10.6039/j.issn.1001-0408.2024.03.08.
SHEN Chengying,LUO Zhong,ZHANG Pei,et al.Study on the antifungal activity and mechanism of Huangqin decoction against Trichophyton mentagrophytes[J].ZHONGGUO YAOFANG,2024,35(03):311-315. DOI： 10.6039/j.issn.1001-0408.2024.03.08.
摘要
研究黄芩汤（HQD）的抗须癣毛癣菌活性及作用机制。
方法
2
通过测定最小抑菌浓度（MIC）、最小杀菌浓度（MFC）、菌丝长度、孢子萌发率、生物量和观察菌丝超微结构评价HQD的抗须癣毛癣菌活性；通过山梨醇保护实验检测HQD对须癣毛癣菌细胞壁的影响；通过测定麦角固醇含量和角鲨烯环氧酶（SE）、羊毛甾醇14
α
-去甲基化酶（CYP51）的活性考察HQD对须癣毛癣菌细胞膜的影响；通过测定线粒体中苹果酸脱氢酶（MDH）、琥珀酸脱氢酶（SDH）及ATP酶（包括钠钾ATP酶、钙镁ATP酶和总ATP酶）的活性考察HQD对须癣毛癣菌线粒体的影响。
结果
2
HQD对须癣毛癣菌具有显著的抑菌活性，MIC、MFC值分别为3.13、25 mg/mL。经HQD干预后，须癣毛癣菌菌丝长度均显著缩短（
P
＜0.05）；孢子萌发率、生物量、细胞膜中麦角固醇含量和SE、CYP51活性以及线粒体中MDH、SDH、各种ATP酶的活性均显著降低（
P
＜0.05）；细胞结构受到了一定程度的破坏，但细胞壁的完整性没有受影响。
结论
2
HQD具有显著的抗须癣毛癣菌活性，其作用机制与降低细胞膜中麦角固醇含量和SE、CYP51活性以及线粒体相关酶活性有关。
Abstract
OBJECTIVE
2
To study the antifungal activity of Huangqin decoction （HQD） against
Trichophyton mentagrophytes
and explore its mechanism.
METHODS
2
Minimal inhibitory concentration （MIC）， minimal fungicidal concentration （MFC）， mycelial length， spore germination rate， biomass and mycelium ultrastructure observation were performed to evaluate the antifungal activity of HQD against
T. mentagrophytes.
The effects of HQD on the cell wall of
T. mentagrophytes
were detected through sorbitol protection experiment. By measuring the content of ergosterol and the activities of squalene epoxide （SE） and lanosterol 14
α
-demethylase （CYP51）， the activity of HQD on the cell membrane of
T. mentagrophytes
was investigated. The effects of HQD on
T. mentagrophytes
mitochondria were investigated by determining the activities of malate dehydrogenase （MDH）， succinate dehydrogenase （SDH）， and ATPases （including sodium potassium ATPase， calcium magnesium ATPase， and total ATPase）.
RESULTS
2
HQD exhibited significant antifungal activity against
T. mentagrophytes
with MIC of 3.13 mg/mL and MFC of 25 mg/mL. After intervention with HQD， the mycelial length of
T. mentagrophytes
was significantly shortened （
P
＜0.05）； spore germination rate， biomass， the content of ergosterol in the cell membrane， the activities of SE and CYP51 in the cell membrane and MDH， SDH and ATPase in mitochondria were all decreased significantly （
P
＜0.05）； cell structure had been damaged to a certain extent， but the integrity of the cell wall had not been affected.
CONCLUSIONS
2
HQD shows significant antifungal activity against
T. mentagrophytes
， the mechanism of which may be associated with reducing the content of ergosterol in the cell membrane and the activities of SE， CYP51， and mitochondria-related enzymes.
关键词
黄芩汤须癣毛癣菌抗真菌活性作用机制细胞膜线粒体
Keywords
Trichophyton mentagrophytesantifungal activitymechanism of actioncell membranemitochondria
references
DE OLIVEIRA PEREIRA F，GOMES S M，LIMA DA SILVA S，et al. The prevalence of dermatophytoses in Brazil：a systematic review[J]. J Med Microbiol，2021，70（3）：1-12.
CAMPOY S，ADRIO J L. Antifungals[J]. Biochem Pharmacol，2017，133：86-96.
BEN-AMI R，KONTOYIANNIS D P. Resistance to antifungal drugs[J]. Infect Dis Clin North Am，2021，35（2）：279-311.
LI M Y，LI M X，XU N，et al. Effects of Huangqin decoction on ulcerative colitis by targeting estrogen receptor alpha and ameliorating endothelial dysfunction based on system pharmacology[J]. J Ethnopharmacol，2021，271：113886.
CHEN P D，ZHOU X，ZHANG L，et al. Anti-inflamma- tory effects of Huangqin tang extract in mice on ulcerative colitis[J]. J Ethnopharmacol，2015，162：207-214.
WANG J Y，LI R，ZHANG M N，et al. Influence of Huangqin decoction on the immune function and fecal microbiome of chicks after experimental infection with Escherichia coli O78[J]. Sci Rep，2022，12（1）：16632.
沈成英，邓冯沂，侯雄军，等. 黄芩汤对临床常见真菌的体外抑菌效果初步观察[J]. 解放军药学学报，2022，35（6）：479-481，488.
SHEN C Y，DENG F Y，HOU X J，et al. In vitro observation of antifungal effect of Huangqin Decoction against common clinical fungi[J]. Pharm J Chin People’s Liberation Army，2022，35（6）： 479-481，488.
李涛. 黄芩汤物质基础与药代动力学特征研究[D]. 北京：中国中医科学院，2013.
LI T. Study on the chemical basis and pharmacokinetics of Huangqin tang[D].Beijing：China Academy of Chinese Medical Sciences，2013.
DE OLIVEIRA LIMA M I，ARAÚJO DE MEDEIROS A C，SOUZA SILVA K V，et al. Investigation of the antifungal potential of linalool against clinical isolates of flucona-zole resistant Trichophyton rubrum[J]. J Mycol Med，2017，27（2）：195-202.
ZENG H，CHEN X P，LIANG J N. In vitro antifungal activity and mechanism of essential oil from fennel （Foeniculum vulgare L.） on dermatophyte species[J]. J Med Microbiol，2015，64（Pt1）：93-103.
LIN H Q，LIU X P，SHEN Z B，et al. The effect of isoflavaspidic acid PB extracted from Dryopteris fragrans L. Schott on planktonic and biofilm growth of dermatophytes and the possible mechanism of antibiofilm[J]. J Ethnopharmacol，2019，241：111956.
LUO N X，JIN L，YANG C Q，et al. Antifungal activity and potential mechanism of magnoflorine against Trichophyton rubrum[J]. J Antibiot，2021，74（3）：206-214.
BOWMAN S M，FREE S J. The structure and synthesis of the fungal cell wall[J]. Bioessays， 2006，28（8）：799-808.
DE OLIVEIRA PEREIRA F，MENDES J M，DE OLIVEIRA LIMA E. Investigation on mechanism of antifungal activity of eugenol against Trichophyton rubrum[J]. Med Mycol，2013，51（5）：507-513.
ZHANG Z S，LIU X P，SHEN Z B，et al. Isoflavaspidic acid PB extracted from Dryopteris fragrans L. Schott inhibits Trichophyton rubrum growth via membrane permeability alternation and ergosterol biosynthesis disruption[J]. Biomed Res Int，2022，2022：6230193.
AN Y F，LIU W X，XIE H L，et al. Construction and acti- vity evaluation of novel benzodioxane derivatives as dual-target antifungal inhibitors[J]. Eur J Med Chem，2022，227：113950.
WU X Z，CHENG A X，SUN L M，et al. Plagiochin E，an antifungal bis（bibenzyl），exerts its antifungal activity through mitochondrial dysfunction-induced reactive oxygen species accumulation in Candida albicans[J]. Biochim Biophys Acta，2009，1790（8）：770-777.
SHEKHOVA E，KNIEMEYER O，BRAKHAGE A A. Induction of mitochondrial reactive oxygen species production by itraconazole，terbinafine，and amphotericin B as a mode of action against Aspergillus fumigatus[J]. Antimicrob Agents Chemother，2017，61（11）：e00978-e00917.
NIVOIX Y，LEDOUX M P，HERBRECHT R. Antifungal therapy：new and evolving therapies[J]. Semin Respir Crit Care Med，2020，41（1）：158-174.
SZYMAŃSKI M，CHMIELEWSKA S，CZYŻEWSKA U，et al. Echinocandins：structure， mechanism of action and use in antifungal therapy[J]. J Enzyme Inhib Med Chem，2022，37（1）：876-894.
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