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1.山西中医药大学中药与食品工程学院,山西 晋中 030619
2.山西中医药大学中药炮制山西省重点实验室,山西 晋中 030619
3.东国大学韩医学院,韩国 庆州 38066
硕士研究生。研究方向:中药新产品开发与应用。 E-mail:wj978123@163.com
教授,博士生导师。研究方向:中药炮制现代研究。电话:0351-3179909。E-mail:zhangshuosheng@aliyun.com
纸质出版日期:2024-02-15,
收稿日期:2023-09-06,
修回日期:2024-01-14,
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王娟,吕辰子,赵彩蓉等.秫米提取物对失眠小鼠睡眠的改善作用及机制研究 Δ[J].中国药房,2024,35(03):322-326.
WANG Juan,LYU Chenzi,ZHAO Cairong,et al.Effects and mechanism of Setaria italica extract on improving sleep in insomnia mice[J].ZHONGGUO YAOFANG,2024,35(03):322-326.
王娟,吕辰子,赵彩蓉等.秫米提取物对失眠小鼠睡眠的改善作用及机制研究 Δ[J].中国药房,2024,35(03):322-326. DOI: 10.6039/j.issn.1001-0408.2024.03.10.
WANG Juan,LYU Chenzi,ZHAO Cairong,et al.Effects and mechanism of Setaria italica extract on improving sleep in insomnia mice[J].ZHONGGUO YAOFANG,2024,35(03):322-326. DOI: 10.6039/j.issn.1001-0408.2024.03.10.
目的
2
研究秫米提取物对失眠小鼠的改善作用及可能机制。
方法
2
将小鼠按随机数字表法分为空白组、模型组、阳性对照组(地西泮,2.6 mg/kg)和秫米提取物低、中、高剂量组(1.2、2.4、4.8 g/kg),每组10只。除空白组外,其余各组小鼠均腹腔注射对氯苯丙氨酸(PCPA)建立失眠模型。造模成功后,空白组和模型组小鼠灌胃等体积生理盐水,各给药组小鼠灌胃相应药物,灌胃体积为0.01 mL/g,每天1次,连续7 d。给药结束后,进行旷场实验观察小鼠行为学变化,测定小鼠海马组织中5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HTAA)以及血清中5-HT、脑源性神经营养因子(BDNF)、白细胞介素2(IL-2)、IL-6、B细胞淋巴瘤2(Bcl-2)、Bcl-2相关X蛋白(Bax)含量;测定小鼠海马中磷脂酰肌醇3激酶/蛋白激酶B/核因子κB(PI3K/Akt/NF-κB)信号通路相关蛋白的表达水平。
结果
2
与模型组比较,秫米提取物高剂量组小鼠的总运动时间均显著延长、总静止时间均显著缩短(
P
<0.01),站立次数及修饰次数均显著减少(
P
<0.01);血清中5-HT、BDNF、Bcl-2的含量和Bcl-2/Bax比值均显著升高,IL-2、IL-6、Bax的含量均显著降低(
P
<0.05或
P
<0.01);海马组织中5-HTAA的含量及PI3K、Akt蛋白磷酸化水平均显著升高,NF-κB p65蛋白磷酸化水平显著降低(
P
<0.05)。
结论
2
高剂量秫米提取物对失眠小鼠有较好的改善效果,其作用机制可能与调控PI3K/Akt/NF-κB信号通路有关。
OBJECTIVE
2
To investigate the effects of
Setaria italica
extract on improving insomnia model mice and to explore its potential mechanisms.
METHODS
2
The mice were randomly assigned into blank group, model group, positive control group (diazepam, 2.6 mg/kg), and
S. italica
extract low-dose, medium-dose and high-dose groups (1.2, 2.4, 4.8 g/kg), with 10 mice in each group. Except for the blank group, all other groups received intraperitoneal injection of para-chlorophenylalanine (PCPA) to establish the insomnia model. After modeling, the blank group and model group were given a constant volume of normal saline intragastrically, and administration groups were given relevant medicine intragastrically, with a volume of 0.01 mL/g, once a day, for 7 consecutive days. After the administration, the open-field test was conducted to observe the praxiological changes of mice, and to determine the levels of 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HTAA) in the hippocampal tissue, as well as the contents of 5-HT, brain-derived neurotrophic factor (BDNF), interleukin-2 (IL-2), IL-6, B-cell lymphoma-2 (Bcl-2), and Bcl-2-associated X protein (Bax) in the serum. The expression of phosphoinositide 3-kinase/protein kinase B/nuclear factor-κB (PI3K/Akt/NF-κB) signaling pathway related protein was determined in the hippocampus of mice.
RESULTS
2
Compared with the model group, the total exercise time of mice in
S. italica
extract high-dose group was significantly prolonged, but the total rest time was significantly shortened (
P
<0.01); the number of standing times and modification times were significantly reduced (
P
<0.01). The contents of 5-HT, BDNF, and Bcl-2 in serum, and Bcl-2/Bax were significantly increased, while the contents of IL-2, IL-6, and Bax were significantly reduced (
P
<0.05 or
P
<0.01). The content of 5-HTAA in the hippocampal tissue and the phosphorylation levels of PI3K and Akt proteins were increased significantly, while the phosphorylation level of NF-κB p65 protein was decreased significantly (
P
<0.05).
CONCLUSIONS
2
High-dose of
S. italica
extract demonstrates significant therapeutic effects on insomnia in mice, and the mechanism of which may be associated with the regulation of PI3K/Akt/NF-κB signaling pathway.
秫米失眠神经炎症磷脂酰肌醇3激酶/蛋白激酶B/核因子κB信号通路
insomnianeuroinflammationPI3K/Akt/NF-κB signaling pathway
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