浏览全部资源
扫码关注微信
滕州市中心人民医院输血科,山东 滕州 277599
主管技师。研究方向:输血检验及血液免疫。E-mail:huamin1h@163.com
主管技师。研究方向:医学检验。E-mail:xgjz3e@163.com
纸质出版日期:2024-03-30,
收稿日期:2023-08-24,
修回日期:2024-01-29,
扫 描 看 全 文
华敏,张伟丽.虎杖苷对AML细胞增殖、迁移、侵袭及肿瘤生长的影响 Δ[J].中国药房,2024,35(06):701-706.
HUA Min,ZHANG Weili.Effects of polydatin on the cell proliferation, migration, invasion and tumor growth of AML[J].ZHONGGUO YAOFANG,2024,35(06):701-706.
华敏,张伟丽.虎杖苷对AML细胞增殖、迁移、侵袭及肿瘤生长的影响 Δ[J].中国药房,2024,35(06):701-706. DOI: 10.6039/j.issn.1001-0408.2024.06.11.
HUA Min,ZHANG Weili.Effects of polydatin on the cell proliferation, migration, invasion and tumor growth of AML[J].ZHONGGUO YAOFANG,2024,35(06):701-706. DOI: 10.6039/j.issn.1001-0408.2024.06.11.
目的
2
探讨虎杖苷(PD)对急性髓系白血病(AML)细胞增殖、迁移、侵袭及肿瘤生长的影响。
方法
2
将人AML细胞KG-1分为正常组,低、中、高浓度PD组(10、30、60 μmol/L的PD),SQ22536组[环磷酸腺苷(cAMP)抑制剂,100 μmol/L],高浓度PD和SQ22536联用组(60 μmol/L的PD+100 μmol/L的SQ22536),考察PD对细胞活力、凋亡率、侵袭和迁移能力、cAMP水平和上皮-间充质转化(EMT)相关蛋白、蛋白激酶A(PKA)蛋白表达的影响。以BALB/c裸鼠为对象,通过皮下接种KG-1细胞悬液建立AML裸鼠移植瘤模型,并分为对照组、PD组、SQ22536组、PD+SQ22536组(每组6只),考察PD对其瘤体体积及质量的影响。
结果
2
与正常组细胞或对照组裸鼠比较,PD各浓度组的细胞活力、迁移细胞数、侵袭细胞数和波形蛋白、锌指转录因子Snail蛋白的相对表达量,以及PD组裸鼠的瘤体体积及质量均显著降低,而细胞凋亡率、cAMP水平、PKA和上皮钙黏素的相对表达量均显著升高,且呈浓度依赖性(
P
<0.05);SQ22536对细胞和裸鼠的作用效果与PD相反,并可显著逆转PD的抗肿瘤活性(
P
<0.05)。
结论
2
PD可能通过激活cAMP/PKA信号通路来抑制AML细胞的增殖、迁移、侵袭,诱导细胞凋亡,抑制EMT进程及肿瘤生长,从而发挥抗AML作用。
OBJECTIVE
2
To investigate the effects of polydatin (PD) on cell proliferation, migration, invasion and tumor growth of acute myeloid leukemia (AML).
METHODS
2
Human AML cell KG-1 were divided into normal group, PD low-, medium- and high-concentration groups (10, 30, 60 μmol/L PD), SQ22536 group [cyclic adenosine monophosphate (cAMP) inhibitor, 100 μmol/L], high concentration of PD+SQ22536 group (60 μmol/L PD+100 μmol/L SQ22536). The effects of PD on cell activity, apoptotic rate, invasion and migration ability, cAMP level, the expression of epithelial-mesenchymal transition (EMT) related proteins and protein kinase A (PKA) were investigated. Using BALB/c nude mice as subjects, a transplanted tumor model of AML nude mice was induced by subcutaneous inoculation of KG-1 cell suspension and then divided into control group, PD group, SQ22536 group and PD+SQ22536 group (with 6 mice in each group). The effects of PD on tumor volume and mass were measured.
RESULTS
2
Compared with the normal group or control group, the cell viabilities, the number of migrating cells, the number of invasive cells, the relative expressions of vimentin and Snail as well as the tumor volume and mass were decreased significantly in PD groups, while the apoptotic rates, cAMP levels, the relative expressions of E-cadherin and PKA were significantly increased, with a dose-dependent manner (
P
<0.05). SQ22536 had opposite effects on cells and nude mice compared to PD, and could significantly reverse the anti-tumor activity of PD (
P
<0.05).
CONCLUSIONS
2
PD may inhibit the proliferation, migration, invasion and EMT process of KG-1 cells, induce apoptosis, and inhibit tumor growth, by activating the cAMP/PKA signaling pathway, thereby exerting anti-AML effects.
虎杖苷急性髓系白血病增殖迁移侵袭上皮-间充质转化环磷酸腺苷/蛋白激酶A信号通路
acute myeloid leukemiaproliferationmigrationinvasionepithelial-mesenchymal transitioncAMP/PKA signaling pathway
PELCOVITS A,NIROULA R. Acute myeloid leukemia:a review[J]. R I Med J,2020,103(3):38-40.
SHIMONY S,STAHL M,STONE R M. Acute myeloid leukemia:2023 update on diagnosis,risk-stratification,and management[J]. Am J Hematol,2023,98(3):502-526.
POLLYEA D A,ALTMAN J K,ASSI R,et al. Acute mye-loid leukemia,version 3.2023,NCCN clinical practice guidelines in oncology[J]. J Natl Compr Canc Netw,2023,21(5):503-513.
WANG C M,LUO Y,LU J,et al. Polydatin induces apoptosis and inhibits growth of acute monocytic leukemia cells[J]. J Biochem Mol Toxicol,2016,30(4):200-205.
CESSNA H,BARITAKI S,ZARAVINOS A,et al. The role of RKIP in the regulation of EMT in the tumor microenvironment[J]. Cancers,2022,14(19):4596.
SAMMARCO A,GOMIERO C,BEFFAGNA G,et al. Epithelial-to-mesenchymal transition and phenotypic marker evaluation in human,canine,and feline mammary gland tumors[J]. Animals,2023,13(5):878.
RODRÍGUEZ GONZÁLEZ A,SAHORES A, DÍAZNEBREDA A,et al. MRP4/ABCC4 expression is regulated by histamine in acute myeloid leukemia cells,determining cAMP efflux[J]. FEBS J,2021,288(1):229-243.
LV M,ZHANG X,JIA H,et al. An oncogenic role of miR-142-3p in human T-cell acute lymphoblastic leukemia(T-ALL)by targeting glucocorticoid receptor-α and cAMP/PKA pathways[J]. Leukemia,2012,26(4):769-777.
STEVENS L E,PELUFFO G,QIU X T,et al. JAK-STAT signaling in inflammatory breast cancer enables chemo-therapy-resistant cell states[J]. Cancer Res,2023,83(2):264-284.
HE B,MOREAU R. R-α-lipoic acid and 4-phenylbutyric acid have distinct hypolipidemic mechanisms in hepatic cells[J]. Biomedicines,2020,8(8):289.
LI R,LI J Z,HUANG Y J,et al. Polydatin attenuates diet-induced nonalcoholic steatohepatitis and fibrosis in mice[J]. Int J Biol Sci,2018,14(11):1411-1425.
汪雪峰,陈锋,宋顺德,等. 腺苷酸环化酶抑制剂和激动剂在脂多糖诱导的急性肺损伤中的作用[J]. 中国药理学通报,2017,33(10):1410-1414.
WANG X F,CHEN F,SONG S D,et al. Effect of adeny-late cyclase antagonists and agonist in acute lung injury induced by lipopolysaccharide[J]. Chin Pharmacol Bull,2017,33(10):1410-1414.
KARAMI A,FAKHRI S,KOOSHKI L,et al. Polydatin:pharmacological mechanisms,therapeutic targets,biolo-gical activities,and health benefits[J]. Molecules,2022,27(19):6474.
王春美,祁文静,任艳娇,等. 虎杖苷对THP-1细胞增殖及凋亡的影响及其作用机制研究[J]. 中国当代儿科杂志,2022,24(7):821-825.
WANG C M,QI W J,REN Y J,et al. Effect of polydatin on the proliferation and apoptosis of THP-1 cells and the mechanism[J]. Chin J Contemp Pediatr,2022,24(7):821-825.
WAN G Q,LIU Y H,ZHU J,et al. SLFN5 suppresses cancer cell migration and invasion by inhibiting MT1-MMP expression via Akt/GSK-3β/β-catenin pathway[J]. Cell Signal,2019,59:1-12.
ZHANG R,LI Y C,WANG H T,et al. The regulation of circRNA RNF13/miRNA-1224-5p axis promotes the malignant evolution in acute myeloid leukemia[J]. Biomed Res Int,2020,2020:5654380.
ZHANG H Y,KONG Q B,WANG J,et al. Complex roles of cAMP-PKA-CREB signaling in cancer[J]. Exp Hematol Oncol,2020,9(1):32.
ILLIANO M,CONTE M,SAPIO L,et al. Forskolin sensitizes human acute myeloid leukemia cells to H3K27me2/3 demethylases GSKJ4 inhibitor via protein kinase A[J]. Front Pharmacol,2018,9:792.
0
浏览量
1
下载量
0
CSCD
关联资源
相关文章
相关作者
相关机构