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1.淄博市第一医院内分泌科,山东 淄博 255200
2.山东齐鲁医院青岛院区内分泌科,山东 青岛 266011
3.淄博市第一医院老年医学科,山东 淄博 255200
主治医师,硕士。研究方向:内分泌与代谢疾病。E-mail:812705889@qq.com
主治医师,硕士。研究方向:心血管内科。E-mail:329426742@qq.com
纸质出版日期:2024-06-30,
收稿日期:2023-11-27,
修回日期:2024-05-09,
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王丽娟,刘元涛,田鹏飞.吴茱萸碱对高糖诱导的滋养层细胞增殖、迁移、侵袭的影响及机制 Δ[J].中国药房,2024,35(12):1463-1468.
WANG Lijuan,LIU Yuantao,TIAN Pengfei.Impact of evodiamine on the proliferation, migration and invasion of trophoblastic cells induced by high glucose and its mechanism[J].ZHONGGUO YAOFANG,2024,35(12):1463-1468.
王丽娟,刘元涛,田鹏飞.吴茱萸碱对高糖诱导的滋养层细胞增殖、迁移、侵袭的影响及机制 Δ[J].中国药房,2024,35(12):1463-1468. DOI: 10.6039/j.issn.1001-0408.2024.12.09.
WANG Lijuan,LIU Yuantao,TIAN Pengfei.Impact of evodiamine on the proliferation, migration and invasion of trophoblastic cells induced by high glucose and its mechanism[J].ZHONGGUO YAOFANG,2024,35(12):1463-1468. DOI: 10.6039/j.issn.1001-0408.2024.12.09.
目的
2
基于晚期糖基化终末产物(AGE)/晚期糖基化终末产物受体(RAGE)信号通路探讨吴茱萸碱对高糖诱导的滋养层细胞增殖、迁移、侵袭能力的影响及潜在机制。
方法
2
将人滋养层细胞HTR-8/SVneo分为对照组、高糖组、吴茱萸碱低浓度组(2 μmol/L)、吴茱萸碱高浓度组(4 μmol/L)、pc-NC组(转染pc-NC质粒+4 μmol/L吴茱萸碱)、pc-RAGE组(转染pc-RAGE质粒+4 μmol/L吴茱萸碱)。除对照组外的其余各组细胞均在高糖(25 mmol/L葡萄糖)环境下培养,除对照组、高糖组外的其余各组细胞均转染相应质粒和(或)接受相应药液干预。检测各组细胞的存活率、凋亡率、划痕愈合率、侵袭数,以及AGE、RAGE、核因子κB p65(NF-κB p65)、基质金属蛋白酶2(MMP-2)、MMP-9蛋白及mRNA的表达情况。
结果
2
与对照组比较,高糖组细胞的存活率、划痕愈合率、侵袭数和MMP-2、MMP-9蛋白及mRNA的表达水平均显著降低(
P
<0.05),其凋亡率,AGE、RAGE蛋白及mRNA的表达水平,NF-κB p65 mRNA的表达水平和NF-κB p65蛋白的磷酸化水平均显著升高(
P
<0.05);与高糖组比较,吴茱萸碱低、高浓度组细胞上述指标均显著改善,且高浓度组的效果显著优于低浓度组(
P
<0.05);过表达RAGE会减弱吴茱萸碱对高糖诱导的滋养层细胞上述指标(AGE mRNA及蛋白除外)的改善作用(
P
<0.05)。
结论
2
吴茱萸碱可促进高糖诱导的滋养层细胞增殖、迁移、侵袭,改善其功能损伤,上述作用与抑制AGE/RAGE信号通路有关。
OBJECTIVE
2
To investigate the impact of evodiamine on the proliferation, migration and invasion abilities of trophoblastic cells induced by high glucose and its potential mechanism based on advanced glycation end products (AGE)/receptor for advanced glycation end products (RAGE) signaling pathway.
METHODS
2
Human trophoblastic cells HTR-8/SVneo were divided into control group, high glucose group, evodiamine low-dose group (2 μmol/L), evodiamine high-dose group (4 μmol/L), pc-NC group (transfected with pc-NC plasmid+4 μmol/L evodiamine), and pc-RAGE group (transfected with pc-RAGE plasmid+ 4 μmol/L evodiamine). Cells in all groups except for the control group were cultured in a high sugar (25 mmol/L glucose) environment, and cells in all groups except for the control group and the model group were transfected with the corresponding plasmids and/or received the corresponding drug interventions. The survival rate, apoptotic rate, scratch healing rate, and invasion number, as well as the protein and mRNA expressions of AGE, RAGE, nuclear factor-κB p65 (NF-κB p65), matrix metalloproteinase-2 (MMP-2), and MMP-9 were examined in each group.
RESULTS
2
Compared with the control group, the cell survival rate, scratch healing rate, invasions number, and the mRNA and protein expressions of MMP-2 and MMP-9 in the high glucose group significantly decreased (
P
<0.05), while the apoptotic rate, the mRNA and protein expressions of AGE and RAGE, the mRNA expression of NF-κB p65, and the phosphorylation level of NF-κB p65 protein significantly increased (
P
<0.05); compared with the high glucose group, the above indexes of cells in evodiamine low-dose and high-dose groups were significantly improved, and the effect of the high-dose group was significantly better than that of the low-dose group (
P
<0.
05); overexpression of RAGE attenuated the ameliorative effect of evodiamine on the above indexes in high glucose-induced trophoblast cells (except for AGE mRNA and protein) (
P
<0.05).
CONCLUSIONS
2
Evodiamine can promote the proliferation, migration and invasion of high glucose-induced trophoblast cells and ameliorate their functional impairment, and the above effects are associated with the inhibition of the AGE/RAGE signaling pathway.
吴茱萸碱高糖滋养层细胞增殖迁移侵袭AGE/RAGE信号通路
high glucosetrophoblast cellsproliferationmigrationinvasionAGE/RAGE signaling pathway
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