纳米孔测序在病原微生物检测中的应用专家共识

张述耀; 侯铁英; 黎小妍; 钟诗龙; 伍俊妍; 黄彬; 中国药理学会治疗药物监测研究专业基层委员会; 广东省药学会临床治疗精准用药专家委员会; 《纳米孔测序在病原微生物检测中的应用专家共识》编写组

doi:10.6039/j.issn.1001-0408.2024.14.01

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纳米孔测序在病原微生物检测中的应用专家共识

指南与共识 | 更新时间：2024-07-25

- 纳米孔测序在病原微生物检测中的应用专家共识
- Expert consensuses on the application of nanopore sequencing technology in the detection of pathogenic microorganisms
- 中国药房 2024年35卷第14期页码：1673-1682
- 作者机构：
  
  1.暨南大学附属广州红十字会医院临床精准治疗中心，广州;510240
  2.华中科技大学协和深圳医院（南山医院），广东深圳　518101
  3.中山大学附属第六医院药学部，广州　510510
  4.广东省人民医院药学部，广州　519041
  5.中山大学孙逸仙纪念医院药学部，广州　510120
  6.中山大学附属第一医院检验科，广州　510080
- 作者简介：
  
  主任药师，博士生导师，博士。研究方向：药物基因组学、微生物药学。E-mail：shuyao0754@qq.com
  a 通信作者主任医师，博士生导师，博士。研究方向：微生物医学。E-mail：houtieying001@126.com
  b 通信作者主任药师，博士。研究方向：药理学、微生物药学。E-mail：lxyzzj@126.com
- 基金信息：
  
  广东省科技创新战略专项（“大专项+任务清单”）项目(STKJ202209072);广东省基础与应用基础研究基金自然科学基金-面上项目(2024A1515010633);浙江省科技计划项目“尖兵”“领雁”研发攻关计划(2023C03068);中国红十字基金会医学赋能公益专项(HSZH202200909);毕节市“揭榜挂帅”重大专项(毕科合重大专项〔2023〕2-2号)
- DOI：10.6039/j.issn.1001-0408.2024.14.01
  中图分类号： R446.5
- 纸质出版日期：2024-07-30，
  
  收稿日期：2024-01-26，
  
  修回日期：2024-06-20，
- 稿件说明：
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张述耀,侯铁英,黎小妍等.纳米孔测序在病原微生物检测中的应用专家共识[J].中国药房,2024,35(14):1673-1682.

ZHANG Shuyao,HOU Tieying,LI Xiaoyan,et al.Expert consensuses on the application of nanopore sequencing technology in the detection of pathogenic microorganisms[J].ZHONGGUO YAOFANG,2024,35(14):1673-1682.
张述耀,侯铁英,黎小妍等.纳米孔测序在病原微生物检测中的应用专家共识[J].中国药房,2024,35(14):1673-1682. DOI： 10.6039/j.issn.1001-0408.2024.14.01.

ZHANG Shuyao,HOU Tieying,LI Xiaoyan,et al.Expert consensuses on the application of nanopore sequencing technology in the detection of pathogenic microorganisms[J].ZHONGGUO YAOFANG,2024,35(14):1673-1682. DOI： 10.6039/j.issn.1001-0408.2024.14.01.

摘要

目的

提高危重症感染性疾病的诊断及救治水平，规范纳米孔测序的临床应用，促进该技术的良性发展。

方法

由中国药理学会治疗药物监测研究专业基层委员会和广东省药学会临床治疗精准用药专家委员会发起并组织多学科专家，采用名义群体法讨论确定共识编写大纲，形成共识初稿；采用德尔菲法进行专家咨询，对专家意见进行分析和修订，形成共识。结果与

结论

最终制定的《纳米孔测序在病原微生物检测中的应用专家共识》涵盖了靶向测序、宏基因组测序和全基因组测序等方向，对纳米孔测序的样本采集与保存、检测过程、生物信息学分析、报告解读等全流程进行了规范，并对其中的关键问题给出了推荐意见。

Abstract

OBJECTIVE

To improve the diagnosis and treatment level of critically ill infectious diseases， standardize the clinical application of nanopore sequencing and promote the sound development of the technology.

METHODS

Division of Therapeutic Drug Monitoring of Chinese Pharmacological Society and Expert Committee of Precision Medicine for Clinical Treatment of Guangdong Pharmaceutical Association initiated and organized multidisciplinary experts to discuss and determine the consensus writing outline by using the nominal group method， forming a preliminary consensus draft； expert consultation was performed by using Delphi method， and then experts’ opinions were analyzed and revised to form consensus.

RESULTS &CONCLUSIONS

Consensuses of Experts on the Application of Nanopore Sequencing Technology in the Detection of Pathogenic Microorganisms

covers targeted sequencing， metagenomic sequencing and whole genome sequencing， and is standardized in terms of sample collection and storage， detection process， bioinformatics analysis and report interpretation； the recommendations are provided for the key issues.

关键词

第三代测序纳米孔测序病原微生物危重症感染临床检验微生物学诊断

Keywords

nanopore sequencingpathogenic microorganismscritical infectionclinical laboratory testsmicrobiological diagnosis

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