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1.新疆医科大学药学院,乌鲁木齐 830011
2.新疆医科大学第一附属医院药学部,乌鲁木齐 830054
硕士研究生。研究方向:中药质量标准。E-mail:gxy121225@gmail.com
教授,硕士生导师,博士。研究方向:中药(民族药)物质基础。E-mail:maguizhi000@126.com
纸质出版日期:2024-07-30,
收稿日期:2024-02-21,
修回日期:2024-06-13,
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郭晓宇,滕亮,张晓雪等.包尔胡特果实的化学成分分析及综合质量评价 Δ[J].中国药房,2024,35(14):1715-1721.
GUO Xiaoyu,TENG Liang,ZHANG Xiaoxue,et al.Analysis of chemical constituents and comprehensive quality evaluation of Rhamni Songoricae Fructus[J].ZHONGGUO YAOFANG,2024,35(14):1715-1721.
郭晓宇,滕亮,张晓雪等.包尔胡特果实的化学成分分析及综合质量评价 Δ[J].中国药房,2024,35(14):1715-1721. DOI: 10.6039/j.issn.1001-0408.2024.14.07.
GUO Xiaoyu,TENG Liang,ZHANG Xiaoxue,et al.Analysis of chemical constituents and comprehensive quality evaluation of Rhamni Songoricae Fructus[J].ZHONGGUO YAOFANG,2024,35(14):1715-1721. DOI: 10.6039/j.issn.1001-0408.2024.14.07.
目的
2
鉴定包尔胡特果实的化学成分,并建立其指纹图谱和同时测定4种成分含量的方法,以综合评价包尔胡特果实的质量。
方法
2
采用超高效液相色谱四极杆-飞行时间串联质谱法鉴定包尔胡特果实中的化学成分。采用高效液相色谱(HPLC)法建立15批包尔胡特果实(编号S1~S15)的指纹图谱,并采用SPSS 26.0与SIMCA 14.1软件进行化学计量学分析;同法测定槲皮素、山柰酚、山柰素、大黄素的含量。
结果
2
包尔胡特果实中共鉴定出35种化合物,包括28种黄酮类成分、5种蒽醌类成分、2种有机酸类成分;其HPLC指纹图谱中共标定19个共有峰,指认出槲皮素、山柰酚、山柰素和大黄素;15批样品的HPLC指纹图谱与对照指纹图谱的相似度均大于0.9。聚类分析结果显示,15批样品聚为2类,其中S1~S5、S7、S9为一类,其余样品为一类,与主成分分析结果相同;正交偏最小二乘法-判别分析结果显示,2、6、1、11(槲皮素)、3、14、8、10、19(大黄素)、5号峰的变量重要性投影值均大于1。槲皮素、山柰酚、山柰素、大黄素的含量分别为0.710~10.478、0.236~0.660、0.334~3.039、0.261~0.504 mg/g。
结论
2
所建化学成分鉴定方法、指纹图谱和含量测定方法简便可行、重复性好,结合化学计量学分析可用于包尔胡特果实质量的综合评价。
OBJECTIVE
2
To identify the chemical constituents of Rhamni Songoricae Fructus and to establish their fingerprints and the method for simultaneous determination of four constituents to comprehensively evaluate the quality of Rhamni Songoricae Fructus.
METHODS
2
The chemical constituents in Rhamni Songoricae Fructus were qualitatively analyzed by ultra-performance liquid chromatography quadrupole-time-of-flight tandem mass spectrometry. The fingerprints of 15 batches of Rhamni Songoricae Fructus were established by HPLC and chemometric analysis was performed by using SPSS 26.0 and SIMCA 14.1 software; the contents of quercetin, kaempferol, kaempferide and emodin were determined by the same method.
RESULTS
2
A total of 35 constituents were identified, including 28 kinds of flavonoids, 5 kinds of anthraquinones and 2 kinds of organic acids. A total of 19 common peaks were identified in the HPLC fingerprints, recognizing quercetin, kaempferol, kaempferide and emodin. The similarities between HPLC fingerprints of 15 batches of samples and control chromatograms were greater than 0.9. The results of cluster analysis showed that 15 batches of samples were divided into 2 classes, of which S1-S5, S7 and S9 were one class and the rest were one class, similar to the results of principal component analysis. The results of the orthogonal partial least squares-discriminant analysis showed that the variable importance projections of peaks No. 2, 6, 1, 11 (quercetin), 3, 14, 8, 10, 19 (emodin), 5 were all greater than 1. The contents of quercetin, kaempferol, kaempferide and emodin ranged from 0.710 to 10.478 mg/g, 0.236 to 0.660 mg/g, 0.334 to 3.039 mg/g, and 0.261 to 0.504 mg/g.
CONCLUSIONS
2
The constructed chemical constituent identification, fingerprint and content determination methods are simple, feasible and reproducible, which combined with chemometric analysis can be used for comprehensive evaluation of the quality of Rhamni Songoricae Fructus.
包尔胡特果实UPLC-QTOF-MSE指纹图谱含量测定化学成分质量评价
UPLC-QTOF-MSEfingerprintcontent determinationchemical constituentsquality evaluation
叶静. 包尔胡特提取物对高脂血症大鼠调脂及抗氧化作用的研究[D]. 乌鲁木齐:新疆医科大学,2013.
YE J. The studies of regulating blood lipid and antioxidant of the Kazakh medicine Rhamnus songorica extract on hyperlipidemia rats[D].Urumqi:Xinjiang Medical University,2013.
厉彦翔. 哈药包尔胡特提取物对大鼠酒精性肝病的作用[D]. 乌鲁木齐:新疆医科大学,2012.
LI Y X. Effect of the Kazakh medicine Rhamnus songo-rica extract on rats with alcoholic liver disease[D].Urumqi:Xinjiang Medical University,2012.
新疆维吾尔自治区药品监督管理局.新疆中药维吾尔药饮片炮制规范:2020年版包尔胡特果实[EB/OL].(2020-11-16)[2022-09-10].https://db.ouryao.com/dfbz/view.php?bookid=xjzywweyyppzgf2020&idx=45https://db.ouryao.com/dfbz/view.php?bookid=xjzywweyyppzgf2020&idx=45.
Xinjiang Uygur Autonomous Region Drug Administration. Processing specification for Xinjiang traditional Chinese medicine and Uyghur medicinal pieces: 2020 edition Rhamni Songoricae Fructus[EB/OL].(2020-11-16)[2022-09-10].https://db.ouryao.com/dfbz/view.php?bookid=xjzywweyyppzgf2020&idx=45.https://db.ouryao.com/dfbz/view.php?bookid=xjzywweyyppzgf2020&idx=45.[4] YANGZ R,WANGZ H,TANGJ F,et al. UPLC-QTOF/MSE and bioassay are available approaches for identifying quality fluctuation of Xueshuantong lyophilized powder in clinic[J]. Front Pharmacol,2018,9:633.
郝壮壮,曹宇欣,刘朦朦,等. 基于HPLC指纹图谱及多指标成分测定结合化学计量学的不同商品规格山慈菇质量差异分析[J]. 中草药,2023,54(6):1935-1945.
HAO Z Z,CAO Y X,LIU M M,et al. Quality differences of different commercial specifications of Cremastrae Pseudobulbus Pleiones Pseudobulbus based on HPLC fingerprint,multi-index component determination and chemometrics[J]. Chin Tradit Herb Drugs,2023,54(6):1935-1945.
XU X J,GUO Y L,CHEN M L,et al. Hypoglycemic activities of flowers of Xanthoceras sorbifolia and identification of anti-oxidant components by off-line UPLC-QTOF-MS/MS-free radical scavenging detection[J]. Chin Herb Med,2024,16(1):151-161.
卢立欣,郑磊,李志明,等. 黄花败酱HPLC多组分定量控制及PCA、OPLS-DA联合GRA法综合质量评价[J]. 中国药师,2023,26(12):510-518.
LU L X,ZHENG L,LI Z M,et al. Comprehensive quality evaluation of Patrina scabiosaefolia Fisch based on HPLC multi-component quantitative control and PCA,OPLS-DA combined with gray correlation analysis[J]. China Pharm,2023,26(12):510-518.
李艳荣,段丽颖,魏红,等. 指纹图谱结合一测多评法评价山楂叶质量[J]. 中国药房,2023,34(22):2727-2733.
LI Y R,DUAN L Y,WEI H,et al. Quality evaluation of Crataegus pinnatifida leaves by fingerprint combined with quantitative analysis of multi-components by single-marker[J]. China Pharm,2023,34(22):2727-2733.
ALKANDAHRI M Y,PAMUNGKAS B T,OKTOBA Z,et al. Hepatoprotective effect of kaempferol:a review of the dietary sources,bioavailability,mechanisms of action,and safety[J]. Adv Pharmacol Pharm Sci,2023,2023:1387665.
司丽君,王雪,王林林,等. 槲皮素的抗炎免疫及部分机制研究[J]. 中国医药导报,2021,18(27):26-29,34.
SI L J,WANG X,WANG L L,et al. Research on anti-inflammatory and immune effects of quercetin and its partial mechanism[J]. China Med Her,2021,18(27):26-29,34.
IJAZ M U,AZIZ S,HAMZA A,et al. Curative effects of kaempferide on cadmium-instigated hepatotoxicity in male albino rats[J]. J King Saud Univ Sci,2023,35(8):102885.
YU L Y,ZHAO Y L,ZHAO Y L. Advances in the pharmacological effects and molecular mechanisms of emodin in the treatment of metabolic diseases[J]. Front Pharmacol,2023,14:1240820.
ZHU X J,GUO S Q,ZHANG M Y,et al. Emodin protects against apoptosis and inflammation by regulating reactive oxygen species-mediated NF-κB signaling in interleukin-1β-stimulated human nucleus pulposus cells[J]. Hum Exp Toxicol,2023,42:9603271221138552.
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